Lac Operator Analogues: Bromodeoxyuridine Substitution in the lac Operator Affects the Rate of Dissociation of the lac Repressor
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چکیده
منابع مشابه
Lac operator analogues: bromodeoxyuridine substitution in the lac operator affects the rate of dissociation of the lac repressor.
As measured by a decreased rate of dissociation, lac repressor binds 10-times tighter to 5-bromodeoxyuridine-substituted lac operator than it does to normal lac operator. This result is obtained both in the absence and in the presence of isopropylthiogalactoside, an inducing ligand. These data are significant with regard to the mechanism of sequence-specific protein-DNA interaction, and also su...
متن کاملPhotochemical attachment of lac repressor to bromodeoxyuridine-substituted lac operator by ultraviolet radiation.
The transducing phage lambdah80dlac carries the lac operator, whereas wild-type lambdah80 does not. We find that in high salt (0.18 M KCl), ultraviolet radiation causes the formation of a very stable complex between repressor and 5-bromodeoxyuridine (BrdU)-substituted lambdah80dlac but not to BrdU-lambdah80 DNA. Studies with inducers of the lac operon confirm the specificity of attachment. In l...
متن کاملModeling the Lac repressor-operator assembly: the influence of DNA looping on Lac repressor conformation.
Repression of transcription of the Escherichia coli Lac operon by the Lac repressor (LacR) is accompanied by the simultaneous binding of LacR to two operators and the formation of a DNA loop. A recently developed theory of sequence-dependent DNA elasticity enables one to relate the fine structure of the LacR-DNA complex to a wide range of heretofore-unconnected experimental observations. Here, ...
متن کاملlac Operator nucleosomes. 2. lac Nucleosomes can change conformation to strengthen binding by lac repressor.
We have shown previously that lac repressor binds specifically and quantitatively to lac operator restriction fragments which have been complexed with histones to form artificial nucleosomes (203 base pair restriction fragment) or core particles (144 base pair restriction fragment. We describe here a quantitative method for determining the equilibrium binding affinities of repressor for these l...
متن کاملRadiosensitivity of DNA in a specific protein-DNA complex: the lac repressor-lac operator complex.
PURPOSE To calculate the probability of radiation-induced frank strand breakage (FSB) at each nucleotide in the Escherichia coli lac repressor-lac operator system using a simulation procedure. To compare calculated and experimental results. To asses the contribution of DNA conformational changes and of the masking by the protein to DNA protection by the repressor. MATERIALS AND METHODS Two st...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1972
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.69.9.2574